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recombinant mouse stem cell factor (scf, peprotech # 250-03)  (PeproTech)


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    Structured Review

    PeproTech recombinant mouse stem cell factor (scf, peprotech # 250-03)
    Recombinant Mouse Stem Cell Factor (Scf, Peprotech # 250 03), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+stem+cell+factor+(scf)/murine+stem+cell+factor/bio_rxiv__2025__04__22__649893-312-73-79
    Average 90 stars, based on 1 article reviews
    recombinant mouse stem cell factor (scf, peprotech # 250-03) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Modification:

    Article Title: Topological constraints governing the use of the chicken HS4 chromatin insulator in oncoretrovirus vectors.
    Article Snippet: .. Cells were preinduced at 1 106 cells/ml in Iscove’s modified Dulbecco’s medium (IMDM) containing 10% defined FBS, L-glutamine, sodium pyruvate, nonessential amino acids, antibiotics, 5% interleukin-3 culture supplement (IL-3; Collaborative Biomedical Products, Bedford, MA), 100 ng/ml recombinant human interleukin-6 (IL-6; Sandoz Pharmaceuticals Corp., Hanover, NJ), and 50 units/ml recombinant mouse stem cell factor (SCF; PeproTech Inc., Rocky Hill, NJ). ..

    Article Title: Partial correction of murine beta-thalassemia with a gammaretrovirus vector for human gamma-globin.
    Article Snippet: Several studies have demonstrated that recombinant lentivirus vectors containing extended globin gene expression cassettes and regulatory elements can ameliorate the pathogenic sequela in murine models of β-thalassemia and sickle cell disease.. Similarly promising results have not yet been obtained with recombinant gammaretrovirus vectors.. Of these two vector classes, only gammaretroviruses have been tested extensively in clinical trials, with a proven ability to transduce long-term reconstituting hematopoietic stem cells with an exceedingly low incidence of serious side effects.

    Article Title: DEREGULATION OF DUX4 AND ERG IN ACUTE LYMPHOBLASTIC LEUKEMIA
    Article Snippet: Lineage-negative enrichment and colony assays of murine haematopoietic cells Bone marrow mononuclear cells were harvested from 10-week old Arf −/− mice and labeled with biotin-conjugated lineage antibodies (Ly-6G and Ly-6C, CD11b, CD45R/B220, CD5, TER-119; BD Biosciences, San Jose, CA), followed by incubation with streptavidin coated magnetic beads (Dynabeads M-280 Streptavidin; Thermo Fisher Scientific Inc., Franklin, MA). .. Lineage-negative cells were purified by magnetic separation and cultured for 48 hours in Iscove’s Modified Dulbecco’s Medium/20% fetal bovine serum supplemented with penicillin-streptomycin, L-glutamine, recombinant mouse stem cell factor (SCF; 50 ng/ml), FLT-3 ligand (40 ng/ml), IL-6 (30 ng/ml), IL-3 (20 ng/ml), and IL-7 (10 ng/ml) (Peprotech, Rocky Hill, NJ). .. Cells were infected on RetroNectin-coated plates for 48 hours (Takara Bio Inc., Shiga, Japan) with MSCV-IRES-GFP (MIG) and MSCV-IRES-RFP (MIR) retroviruses expressing the following: wild-type, NRAS G12D , ERG wt , ERG e6alt , tricistronic vector of ERG wt and NRAS G12D , and ERG e6alt and NRAS G12D for MIG; wild-type and IK6 (isoform derived from IKZF1 deletion of exon4-7) for MIR.

    Recombinant:

    Article Title: Topological constraints governing the use of the chicken HS4 chromatin insulator in oncoretrovirus vectors.
    Article Snippet: .. Cells were preinduced at 1 106 cells/ml in Iscove’s modified Dulbecco’s medium (IMDM) containing 10% defined FBS, L-glutamine, sodium pyruvate, nonessential amino acids, antibiotics, 5% interleukin-3 culture supplement (IL-3; Collaborative Biomedical Products, Bedford, MA), 100 ng/ml recombinant human interleukin-6 (IL-6; Sandoz Pharmaceuticals Corp., Hanover, NJ), and 50 units/ml recombinant mouse stem cell factor (SCF; PeproTech Inc., Rocky Hill, NJ). ..

    Article Title: Cytoskeletal Protein 4.1R Is a Positive Regulator of the FcεRI Signaling and Chemotaxis in Mast Cells
    Article Snippet: .. The cells were cultured for 8–12 weeks in RPMI-1640 culture medium supplemented with 10% fetal calf serum, minimum essential medium non-essential amino acids, 0.7 mM sodium pyruvate, 2.5 mM L-glutamine, 12 mM D-glucose, antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin), 71 μM 2-mercaptoethanol, recombinant mouse stem cell factor (SCF; 15 ng/ml, PeproTech EC), and recombinant mouse IL-3 (15 ng/ml, PeproTech EC). ..

    Article Title: Partial correction of murine beta-thalassemia with a gammaretrovirus vector for human gamma-globin.
    Article Snippet: Several studies have demonstrated that recombinant lentivirus vectors containing extended globin gene expression cassettes and regulatory elements can ameliorate the pathogenic sequela in murine models of β-thalassemia and sickle cell disease.. Similarly promising results have not yet been obtained with recombinant gammaretrovirus vectors.. Of these two vector classes, only gammaretroviruses have been tested extensively in clinical trials, with a proven ability to transduce long-term reconstituting hematopoietic stem cells with an exceedingly low incidence of serious side effects.

    Article Title: New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling
    Article Snippet: .. Cells were cultured in RPMI-1640 medium 113 supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 71 μM 2-mercaptoethanol, 114 minimum essential medium (MEM) non-essential amino acids, 0.7 mM sodium pyruvate, 2.5 115 mM L-glutamine, 12 mM D-glucose, recombinant mouse stem cell factor (SCF; 15 ng/ml, 116 PeproTech EC), mouse recombinant interleukin (IL)-3 (20 ng/ml, PeproTech EC), and 10% fetal 117 calf serum (FCS). ..

    Article Title: DEREGULATION OF DUX4 AND ERG IN ACUTE LYMPHOBLASTIC LEUKEMIA
    Article Snippet: Lineage-negative enrichment and colony assays of murine haematopoietic cells Bone marrow mononuclear cells were harvested from 10-week old Arf −/− mice and labeled with biotin-conjugated lineage antibodies (Ly-6G and Ly-6C, CD11b, CD45R/B220, CD5, TER-119; BD Biosciences, San Jose, CA), followed by incubation with streptavidin coated magnetic beads (Dynabeads M-280 Streptavidin; Thermo Fisher Scientific Inc., Franklin, MA). .. Lineage-negative cells were purified by magnetic separation and cultured for 48 hours in Iscove’s Modified Dulbecco’s Medium/20% fetal bovine serum supplemented with penicillin-streptomycin, L-glutamine, recombinant mouse stem cell factor (SCF; 50 ng/ml), FLT-3 ligand (40 ng/ml), IL-6 (30 ng/ml), IL-3 (20 ng/ml), and IL-7 (10 ng/ml) (Peprotech, Rocky Hill, NJ). .. Cells were infected on RetroNectin-coated plates for 48 hours (Takara Bio Inc., Shiga, Japan) with MSCV-IRES-GFP (MIG) and MSCV-IRES-RFP (MIR) retroviruses expressing the following: wild-type, NRAS G12D , ERG wt , ERG e6alt , tricistronic vector of ERG wt and NRAS G12D , and ERG e6alt and NRAS G12D for MIG; wild-type and IK6 (isoform derived from IKZF1 deletion of exon4-7) for MIR.

    Article Title: Single-cell resolution reveals RalA GTPase expanding hematopoietic stem cells and facilitating of BCR-ABL1-driven leukemogenesis in a CRISPR/Cas9 gene editing mouse model
    Article Snippet: .. BMCs were cultured in IMDM with GlutaMAX including 20% FBS, recombinant mouse stem cell factor (SCF, 50 ng/mL) (PeproTech, USA), 2-mercaptoethanol (50 μM), 1% penicillin/streptomycin (PS), recombinant mouse interleukin (IL)-3 (10 ng/mL), and IL-6 (25 ng/mL) or subjected to retroviral transformation with BCR-ABL1. ..

    Article Title: Transmembrane Adaptor Protein PAG/CBP Is Involved in both Positive and Negative Regulation of Mast Cell Signaling
    Article Snippet: .. Cells were cultured in RPMI 1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 71 μM 2-mercaptoethanol, minimum essential medium (MEM) nonessential amino acids, 0.7 mM sodium pyruvate, 2.5 mM l -glutamine, 12 mM d -glucose, recombinant mouse stem cell factor (SCF; 20 ng/ml; PeproTech EC), mouse recombinant interleukin-3 (IL-3; 20 ng/ml; PeproTech EC), and 10% fetal calf serum (FCS). ..

    Cell Culture:

    Article Title: Cytoskeletal Protein 4.1R Is a Positive Regulator of the FcεRI Signaling and Chemotaxis in Mast Cells
    Article Snippet: .. The cells were cultured for 8–12 weeks in RPMI-1640 culture medium supplemented with 10% fetal calf serum, minimum essential medium non-essential amino acids, 0.7 mM sodium pyruvate, 2.5 mM L-glutamine, 12 mM D-glucose, antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin), 71 μM 2-mercaptoethanol, recombinant mouse stem cell factor (SCF; 15 ng/ml, PeproTech EC), and recombinant mouse IL-3 (15 ng/ml, PeproTech EC). ..

    Article Title: New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling
    Article Snippet: .. Cells were cultured in RPMI-1640 medium 113 supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 71 μM 2-mercaptoethanol, 114 minimum essential medium (MEM) non-essential amino acids, 0.7 mM sodium pyruvate, 2.5 115 mM L-glutamine, 12 mM D-glucose, recombinant mouse stem cell factor (SCF; 15 ng/ml, 116 PeproTech EC), mouse recombinant interleukin (IL)-3 (20 ng/ml, PeproTech EC), and 10% fetal 117 calf serum (FCS). ..

    Article Title: DEREGULATION OF DUX4 AND ERG IN ACUTE LYMPHOBLASTIC LEUKEMIA
    Article Snippet: Lineage-negative enrichment and colony assays of murine haematopoietic cells Bone marrow mononuclear cells were harvested from 10-week old Arf −/− mice and labeled with biotin-conjugated lineage antibodies (Ly-6G and Ly-6C, CD11b, CD45R/B220, CD5, TER-119; BD Biosciences, San Jose, CA), followed by incubation with streptavidin coated magnetic beads (Dynabeads M-280 Streptavidin; Thermo Fisher Scientific Inc., Franklin, MA). .. Lineage-negative cells were purified by magnetic separation and cultured for 48 hours in Iscove’s Modified Dulbecco’s Medium/20% fetal bovine serum supplemented with penicillin-streptomycin, L-glutamine, recombinant mouse stem cell factor (SCF; 50 ng/ml), FLT-3 ligand (40 ng/ml), IL-6 (30 ng/ml), IL-3 (20 ng/ml), and IL-7 (10 ng/ml) (Peprotech, Rocky Hill, NJ). .. Cells were infected on RetroNectin-coated plates for 48 hours (Takara Bio Inc., Shiga, Japan) with MSCV-IRES-GFP (MIG) and MSCV-IRES-RFP (MIR) retroviruses expressing the following: wild-type, NRAS G12D , ERG wt , ERG e6alt , tricistronic vector of ERG wt and NRAS G12D , and ERG e6alt and NRAS G12D for MIG; wild-type and IK6 (isoform derived from IKZF1 deletion of exon4-7) for MIR.

    Article Title: Single-cell resolution reveals RalA GTPase expanding hematopoietic stem cells and facilitating of BCR-ABL1-driven leukemogenesis in a CRISPR/Cas9 gene editing mouse model
    Article Snippet: .. BMCs were cultured in IMDM with GlutaMAX including 20% FBS, recombinant mouse stem cell factor (SCF, 50 ng/mL) (PeproTech, USA), 2-mercaptoethanol (50 μM), 1% penicillin/streptomycin (PS), recombinant mouse interleukin (IL)-3 (10 ng/mL), and IL-6 (25 ng/mL) or subjected to retroviral transformation with BCR-ABL1. ..

    Article Title: Transmembrane Adaptor Protein PAG/CBP Is Involved in both Positive and Negative Regulation of Mast Cell Signaling
    Article Snippet: .. Cells were cultured in RPMI 1640 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 71 μM 2-mercaptoethanol, minimum essential medium (MEM) nonessential amino acids, 0.7 mM sodium pyruvate, 2.5 mM l -glutamine, 12 mM d -glucose, recombinant mouse stem cell factor (SCF; 20 ng/ml; PeproTech EC), mouse recombinant interleukin-3 (IL-3; 20 ng/ml; PeproTech EC), and 10% fetal calf serum (FCS). ..

    Purification:

    Article Title: DEREGULATION OF DUX4 AND ERG IN ACUTE LYMPHOBLASTIC LEUKEMIA
    Article Snippet: Lineage-negative enrichment and colony assays of murine haematopoietic cells Bone marrow mononuclear cells were harvested from 10-week old Arf −/− mice and labeled with biotin-conjugated lineage antibodies (Ly-6G and Ly-6C, CD11b, CD45R/B220, CD5, TER-119; BD Biosciences, San Jose, CA), followed by incubation with streptavidin coated magnetic beads (Dynabeads M-280 Streptavidin; Thermo Fisher Scientific Inc., Franklin, MA). .. Lineage-negative cells were purified by magnetic separation and cultured for 48 hours in Iscove’s Modified Dulbecco’s Medium/20% fetal bovine serum supplemented with penicillin-streptomycin, L-glutamine, recombinant mouse stem cell factor (SCF; 50 ng/ml), FLT-3 ligand (40 ng/ml), IL-6 (30 ng/ml), IL-3 (20 ng/ml), and IL-7 (10 ng/ml) (Peprotech, Rocky Hill, NJ). .. Cells were infected on RetroNectin-coated plates for 48 hours (Takara Bio Inc., Shiga, Japan) with MSCV-IRES-GFP (MIG) and MSCV-IRES-RFP (MIR) retroviruses expressing the following: wild-type, NRAS G12D , ERG wt , ERG e6alt , tricistronic vector of ERG wt and NRAS G12D , and ERG e6alt and NRAS G12D for MIG; wild-type and IK6 (isoform derived from IKZF1 deletion of exon4-7) for MIR.

    Retroviral:

    Article Title: Single-cell resolution reveals RalA GTPase expanding hematopoietic stem cells and facilitating of BCR-ABL1-driven leukemogenesis in a CRISPR/Cas9 gene editing mouse model
    Article Snippet: .. BMCs were cultured in IMDM with GlutaMAX including 20% FBS, recombinant mouse stem cell factor (SCF, 50 ng/mL) (PeproTech, USA), 2-mercaptoethanol (50 μM), 1% penicillin/streptomycin (PS), recombinant mouse interleukin (IL)-3 (10 ng/mL), and IL-6 (25 ng/mL) or subjected to retroviral transformation with BCR-ABL1. ..

    Transformation Assay:

    Article Title: Single-cell resolution reveals RalA GTPase expanding hematopoietic stem cells and facilitating of BCR-ABL1-driven leukemogenesis in a CRISPR/Cas9 gene editing mouse model
    Article Snippet: .. BMCs were cultured in IMDM with GlutaMAX including 20% FBS, recombinant mouse stem cell factor (SCF, 50 ng/mL) (PeproTech, USA), 2-mercaptoethanol (50 μM), 1% penicillin/streptomycin (PS), recombinant mouse interleukin (IL)-3 (10 ng/mL), and IL-6 (25 ng/mL) or subjected to retroviral transformation with BCR-ABL1. ..



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